Article Figures & Data
Figures
- FIGURE 1.
Disposable cassette fluidic paths used for 3-reactor syntheses. (A) Top view with fluidic connections inside (dashed) and outside (solid) each cassette, including purification cartridges. Side view of cassettes display reactor in Add position (B), where fluid is delivered into reaction vessel, and Transfer position (C), where fluid is transferred out of reaction vessel. SPE = solid phase extraction; QMA = quarternary methylammonium.
- FIGURE 2.
Examples of HPLC chromatograms. (A and B) 18F-FLT semipreparative HPLC (A) and analytic HPLC of purified sample with coinjection of standard (B). (C and D) 18F-FHBG semipreparative HPLC (C) and analytic HPLC of purified sample with coinjection of standard (D). Chromatography conditions are found in Supplemental Table 2. *Radioactive peak of interest. AU = absorbance units; CPS = counts per second; UV = ultraviolet.
- FIGURE 3.
Small-animal PET imaging using same-day sequentially synthesized PET tracers on single automated radiosynthesizer. Sample images are shown for 18F-FLT (A), 18F-FDG (B), and 18F-fallypride (C). Scale for percentage injected dose per gram (%ID/g) is listed for each image. BL = bladder; BM = bone marrow; GB = gallbladder; GI = gastrointestinal tract; HR = heart; KD = kidneys; LG = lacrimal glands; ST = striatum; TM = A431 implanted tumor.
- FIGURE 4.
Chromatograms of purified and reformulated products. (A) d-18F-FAC analytic HPLC with coinjection of standard. (B) 18F-FDG radio-TLC. Chromatography conditions are found in Supplemental Table 2. AU = absorbance units; CPS = counts per second; UV = ultraviolet.
Tables
Specific activity† Tracer Decay-corrected radiochemical yield (%) Synthesis duration (min)* GBq/μmol Ci/μmol d-18F-FAC 31 ± 4 (n = 11) 163 37–44 1.0–1.2 l-18F-FMAU 49 ± 7 (n = 7) 170 100–170 2.7–4.6 d-18F-FEAU (3-reactor) 39 ± 4 (n = 3) 180 3.8–5.1‡ 0.10–0.14 d-18F-FEAU (1-reactor) 28 ± 4 (n = 3) 140 7.4–20‡ 0.20–0.53 18F-FDG 70 ± 9 (n = 3) 38 NA 18F-FLT 69 ± 3 (n = 5) 65 67–481 1.8–13 18F-fallypride 66 ± 8 (n = 6) 56 15–78‡ 0.4–2.1 18F-FHBG 11 ± 2 (n = 3) 87 92–189 2.5–5.1 18F-SFB 69 ± 8 (n = 6) 78 63 1.7 ↵* Synthesis duration was defined from start of synthesis to end of synthesis, including purification but not including reformulation.
↵† Range of specific activities measured at time of injection into analytic HPLC.
↵‡ Starting activity was lower than average and ranged from 1.3 to 1.9 GBq (35–50 mCi).
NA = not applicable.
Radiochemical yields are mean ± SD.
Supplemental Data
Files in this Data Supplement:
