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Research ArticleRadiopharmacy

Miniaturized Radiochemical Purity Testing for 99mTc-HMPAO, 99mTc-HMDP, and 99mTc-Tetrofosmin

Ana de la Fuente, Roberta Zanca, Roberto Boni, Angela G. Cataldi, Martina Sollini, Elena Lazzeri, Giuliano Mariani and Paola A. Erba
Journal of Nuclear Medicine Technology September 2017, 45 (3) 236-240; DOI: https://doi.org/10.2967/jnmt.115.154484
Ana de la Fuente
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Roberta Zanca
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Roberto Boni
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Angela G. Cataldi
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Martina Sollini
2Nuclear Medicine Unit, Department of Oncology and Advanced Technology, “Arcispedale Santa Maria Nuova”–IRCCS, Reggio Emilia, Italy
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Elena Lazzeri
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Giuliano Mariani
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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Paola A. Erba
1Regional Center of Nuclear Medicine, University of Pisa, Pisa, Italy; and
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  • FIGURE 1.
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    FIGURE 1.

    Chromatography diagrams of alternative (A) and standard (B) methods for radiochemical purity testing of radiopharmaceuticals.

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    FIGURE 2.

    Radiochemical purity of 99mTc-HMPAO (left), 99mTc-HMDP (middle), and 99mTc-tetrofosmin (right), obtained by standard and miniaturized strips in commercial reconstituted samples. Correlation coefficients were, respectively, 0.97, 0.93, and 0.86 (with P = 0.0013, P < 0.0001, and P < 0.0001, respectively).

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    FIGURE 3.

    ITLC-SA with acetone:dichloromethane (85:65) of batch of 99mTc-tetrofosmin: (A) Manufacturer's method: radiochemical purity, 94.4%. (B) Miniaturized method: radiochemical purity, 93.7%. Impurities are HRTc (peak 1), unknown impurities (peaks 2 and 3), 99mTc-tetrofosmin (peak 4), and 99mTcO4− (peak 5), accounting for 2% of total radioactivity.

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    TABLE 1

    Rf Ranges for Peaks of Most Common 99mTc Components in Radiopharmaceutical Preparations

    AgentComponentStandard stripsMiniaturized strips
    99mTc-HMPAONaClMEKNaClMEK
    99mTc-pertechnetate0.8–10.8–10.8–10.8–1
    99mTc-albumin0–0.40–0.20–0.20–0.2
    99mTc-HMPAO hydrophilic0–0.40–0.20–0.20–0.2
    99mTc-HMPAO lipophilic0–0.40.8–10–0.20.8–1
    99mTc-HMDPNaClMeOH/H2O*NaClMeOH/H2O
    99mTc-pertechnetate0–0.150.4–10–0.150.5–1
    99mTc-albumin colloid0–0.150–0.40–0.150–0.5
    99mTc-HMDP0.4–10–0.40.4–10.5–1
    99mTc-tetrofosminAcO/DCM†AcO/DCM
    99mTc-pertechnetate0.8–10.9–1
    99mTc-albumin colloid0–0.250–0.3
    99mTc-tetrofosmin0.25–0.80.3–0.9
    • ↵* Methanol:water (85:15).

    • ↵† Acetone:dichloromethane (65:35).

    • MEK = methyl ethyl ketone.

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Journal of Nuclear Medicine Technology: 45 (3)
Journal of Nuclear Medicine Technology
Vol. 45, Issue 3
September 1, 2017
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Miniaturized Radiochemical Purity Testing for 99mTc-HMPAO, 99mTc-HMDP, and 99mTc-Tetrofosmin
Ana de la Fuente, Roberta Zanca, Roberto Boni, Angela G. Cataldi, Martina Sollini, Elena Lazzeri, Giuliano Mariani, Paola A. Erba
Journal of Nuclear Medicine Technology Sep 2017, 45 (3) 236-240; DOI: 10.2967/jnmt.115.154484
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Keywords

  • quality control
  • ITLC
  • 99mTc-HMPAO
  • 99mTc-HMDP
  • 99mTc-tetrofosmin
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Miniaturized Radiochemical Purity Testing for 99mTc-HMPAO, 99mTc-HMDP, and 99mTc-Tetrofosmin
Ana de la Fuente, Roberta Zanca, Roberto Boni, Angela G. Cataldi, Martina Sollini, Elena Lazzeri, Giuliano Mariani, Paola A. Erba
Journal of Nuclear Medicine Technology Sep 2017, 45 (3) 236-240; DOI: 10.2967/jnmt.115.154484

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