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Research ArticleStudent Investigations

The Efficacy of Radiolabeling the Albumin in Egg Whites with 99mTc-Sulfur Colloid

Deontae L. Green and Sara L. Johnson
Journal of Nuclear Medicine Technology March 2024, 52 (1) 59-62; DOI: https://doi.org/10.2967/jnmt.123.266794
Deontae L. Green Sr.
1Lakewood Ranch Medical Center, Lakewood Ranch, Florida;
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Sara L. Johnson
2Gurnick Academy of the Medical Arts, Concord, California; and
3Adventist Health University Orlando, Orlando, Florida
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  • FIGURE 1.
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    FIGURE 1.

    Materials used to simulate gastric environment and examine radiolabeling: simulated gastric fluid with HCl and pepsin. Bovine serum albumin was used to evaluate radiolabeling efficacy.

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    FIGURE 2.

    Twelve test tubes with radiolabeled cooked egg whites separated from simulated gastric fluid.

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    TABLE 1.

    Materials Used for This Research

    MaterialQuantity
    99mTc-SC5.92 MBq (160 μCi)
    1% bovine serum albumin0.5 mL
    NaCl15 mL
    HCl and pepsin3.5 mL
    Distilled water50 mL
    Sterile gauze pack1
    Test tubes24
    Test tube rack1
    1,000-mL beakers2
    Hot plate with stirrer1
    Food scale1
    3-mL syringes12
    Liquid egg whites118 mL
    Paper cup2
    Square disposable plastic scale-tray2
    Metal laboratory spatula1
    Foam cup2
    Microwave1
    Dose calibrator1
    Well counter1
    Survey meter1
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    TABLE 2.

    Radiolabeling Efficacy Results for Precooked Sample

    TimeSimulated gastric fluidPrecooked egg whitesRadiolabeling efficacy
    2 h5,78412,656(12,656/18,440) × 100 = 69%
    5,67612,689(12,689/18,365) × 100 = 69%
    5,97412,679(12,679/18,653) × 100 = 68%
    4 h4,54212,628(12,628/17,170) × 100 = 74%
    5,20012,630(12,630/17,830) × 100 = 71%
    5,46712,659(12,659/18,126) × 100 = 70%
    • Mean of precooked sample set is 70%. All measurements are in counts per minute.

    • View popup
    TABLE 3.

    Radiolabeling Efficacy Results for Postcooked Sample

    TimeSimulated gastric fluidPostcooked egg whitesRadiolabeling efficacy
    2 h12,54812,176(12,176/24,724) × 100 = 49%
    11,66610,954(10,954/22,620) × 100 = 48%
    11,16511,358(11,358/23,023) × 100 = 49%
    4 h10,90311,478(11,478/22,381) × 100 = 51%
    11,72912,572(12,572/24,301) × 100 = 52%
    12,19611,973(11,973/24,169) × 100 = 50%
    • Mean of postcooked sample set is 50%. All measurements are in counts per minute.

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Journal of Nuclear Medicine Technology: 52 (1)
Journal of Nuclear Medicine Technology
Vol. 52, Issue 1
March 1, 2024
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The Efficacy of Radiolabeling the Albumin in Egg Whites with 99mTc-Sulfur Colloid
Deontae L. Green, Sara L. Johnson
Journal of Nuclear Medicine Technology Mar 2024, 52 (1) 59-62; DOI: 10.2967/jnmt.123.266794

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The Efficacy of Radiolabeling the Albumin in Egg Whites with 99mTc-Sulfur Colloid
Deontae L. Green, Sara L. Johnson
Journal of Nuclear Medicine Technology Mar 2024, 52 (1) 59-62; DOI: 10.2967/jnmt.123.266794
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