The Presence of Sodium Nitrate in Generator Eluate Decreases the Radiochemical Purity of 99mTc-Sestamibi

Thierry Métayé; Thierry Rosenberg; Joëlle Guilhot; Marie-Hélène Bouin-Pineau; Rémy Perdrisot

doi:10.2967/jnmt.111.101246

Article Figures & Data

Figures

Tables
Additional Files

Download figure
Open in new tab
Download powerpoint
FIGURE 1.
Distribution of RCP values of ^99mTc-sestamibi preparations using ^99mTc-pertechnetate from 3 different generators. Dotted line represents RCP limit (94%) recommended by manufacturer for clinical application. Number of RCP values under 94% is 16, 32, 2, and 0 for CardCis, StamCis, StamGE, and StamCov, respectively. CardCis = Cardiolite kit prepared with ^99mTc eluate from NCWC generator; StamCis = Stamicis kit prepared with ^99mTc eluate from NCWC generator; StamGE = Stamicis kit prepared with ^99mTc eluate from NFDC generator; StamCov = Stamicis kit prepared with ^99mTc eluate from NFWC generator; NS = not significant.
Download figure
Open in new tab
Download powerpoint
FIGURE 2.
Effect of nitrate concentrations on RCP of Stamicis preparations. (A) TLC of ^99mTc-sestamibi using Baker-flex aluminum oxide sheet as stationary phase and absolute ethanol as mobile phase. Dotted line indicates cutting position to determine percentages of ^99mTc-sestamibi and radioimpurity fractions. (B) Nitrate-purity curve of RCP values obtained in A. Data are mean ± SD of triplicate RCP determinations. *P < 0.01. **P ≤ 0.001. A color version of this figure is available as a supplemental file at http://tech.snmjournals.org.
Download figure
Open in new tab
Download powerpoint
FIGURE 3.
Myocardial perfusion images with ^99mTc-sestamibi prepared with wet-column generator eluate containing nitrates (0.05 mg/mL). RCP of Stamicis preparation was 82.8%. Raw data and tomographic slices are presented in upper and lower image series, respectively. Stress images are displayed on top rows and rest images on bottom rows. Sternal region and hepatic dome were used as regions of interest to determine heart-to-lung and heart-to-liver ratios, respectively. Heart-to-lung ratio was 2.2, and heart-to-liver ratio was 0.61. A color version of this figure is available as a supplemental file at http://tech.snmjournals. org.

View popup

TABLE 1

Sample Characteristics of RCP Measurements in Different ^99mTc-Sestamibi Preparations

^99mTc-sestamibi preparation	Preparation number	Median (%)	Minimum (%)	Maximum (%)	Mean (%)	SD (%)
CardCis^*	56	95.25	89.10	97.90	94.93	1.75
StamCis^†	53	93.40	81.30	97.40	92.54	3.55
StamGE^‡	51	96.80	89.60	98.40	96.72	1.31
StamCov^§	23	96.60	94.90	98.90	96.68	0.94

↵* Cardiolite kit prepared with ^99mTc eluate from NCWC generator.
↵† Stamicis kit prepared with ^99mTc eluate from NCWC generator.
↵‡ Stamicis kit prepared with ^99mTc eluate from NFDC generator.
↵§ Stamicis kit prepared with ^99mTc eluate from NFWC generator.

View popup

TABLE 2

Influence of Different Factors on RCP (mean ± SD) of ^99mTc-Sestamibi Preparations

Factor	CardCis^*	StamCis^†	StamGE^‡	StamCov^§	P
Presence vs. absence of nitrate (0.05 mg/mL)		92.54 ± 3.55 92.54 ± 3.55	96.72 ± 1.31	96.68 ± 0.94	<0.0001 <0.0001
Wet vs. dry column (absence of nitrate)			96.72 ± 1.31	96.68 ± 0.94	NS
Kit type	94.93 ± 1.75	92.54 ± 3.55			<0.0001

↵* Cardiolite kit prepared with ^99mTc eluate from NCWC generator.
↵† Stamicis kit prepared with ^99mTc eluate from NCWC generator.
↵‡ Stamicis kit prepared with ^99mTc eluate from NFDC generator.
↵§ Stamicis kit prepared with ^99mTc eluate from NFWC generator.
NS = not significant.

View popup

TABLE 3

Influence of ^99mTc Generator Eluates on RCP Values of Different Radiopharmaceuticals

		NCWC generator		NFDC generator		NFWC generator
Radiopharmaceutical	Method	N	Mean ± SD	N	Mean ± SD	N	Mean ± SD	P
^99mTc-succimer		31	99.77 ± 0.28	19	99.82 ± 0.21	9	99.49 ± 0.99	NS
^99mTc-oxidronate	1	31	97.65 ± 0.70	NT	NT	31	97.54 ± 0.85	NS
	2	31	98.09 ± 1.22	31	98.14 ± 0.91	NT	NT	NS
^99mTc-tetrofosmin	1	31	95.39 ± 1.32	NT	NT	31	94.13 ± 0.89	0.0002
	2	31	98.28 ± 0.54	31	98.14 ± 0.88	NT	NT	NS
^99mTc-exametazime	1	31	90.51 ± 2.25	NT	NT	31	89.51 ± 2.28	0.0532
	2	31	91.33 ± 1.92	31	90.39 ± 2.13	NT	NT	NS
^99mTc-albumin nanocolloid		32	99.88 ± 0.14	22	99.84 ± 0.16	32	99.86 ± 0.18	NS
^99mTc-soluble albumin		35	97.31 ± 4.38	38	97.89 ± 1.36	NT	NT	NS

NT = not tested; NS = not significant.
For ^99mTc-oxidronate, method 1 used ITLC/SG strips from Varian SA and method 2 used ITLC/SG strips from Pall Corp. For ^99mTc-tetrofosmin, method 1 used chromatography paper from Varian SA and method 2 used ITLC/SG strips from Pall Corp. For ^99mTc-exametazime, method 1 used chromatography paper from Varian SA and method 2 used ITLC/SG strips from Pall Corp. P values compared data from NCWC generators with those obtained from NFDC or NFWC generators.