NONINVASIVE STRATEGIES TO IMAGE CARDIOVASCULAR APOPTOSIS
Section snippets
Membrane Changes in Apoptosis
Following activation of proapoptotic caspase enzymes (caspase-8 or caspase-3), the cell is committed to programmed cell death. Coincident with caspase activation there is a rapid rearrangement of the plasma membrane phospholipid structure (Fig. 1). The membrane changes result in the sudden expression of phosphatidylserine (PS), a negatively charged aminophospholipid, which normally is restricted to the inner leaflet of the lipid bilayer, on the cell surface. This is accompanied by a random
IMAGING APOPTOSIS USING RADIOLABELED ANNEXIN V
Radionuclide imaging of apoptotic cell death was initially described by Blankenberg et al6 in 1998, using radiolabeled annexin V as a hot-spot agent. Annexin belongs to a family of proteins that share the common property of calcium dependent binding to membrane bound phosphatidylserine.52 At least 20 different annexins have been identified, of which 10 have been described in mammals.49 Annexin V (MW 36.000, 319 amino acids), initially purified from human placenta,10, 51, belongs to this family
References (67)
- et al.
Quantitative analysis of apoptotic cell death using proton nuclear magnetic resonance spectroscopy
Blood
(1997) - et al.
Radionuclide Imaging of acute lung transplant rejection with annexin V
Chest
(2000) - et al.
Ultrasonic biomicroscopy of viable, dead and apoptotic cells
Ultrasound Med Biol
(1997) - et al.
Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: A flow cytometry study showing a role for free sulfhydryl groups
Blood
(1993) - et al.
High frequency colour flow imaging of the microcirculation
Ultrasound Med Biol
(2000) Technetium-99m labeled myocardial perfusion imaging agents
Semin Nucl Med
(1999)- et al.
Measurement of plasma annexin V by ELISA in the early detection of acute myocardial infarction
Clin Chim Acta
(1996) - et al.
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis
Blood
(1994) - et al.
Ultrastructural and morphometric study of the human heart muscle cell in acute coronary insufficiency
Hum Pathol
(1979) - et al.
Mitochondrial localization and characterization of 99mTc-Sestamibi in heart cells by electron probe X-ray microanalysis and 99Tc-NMR spectroscopy
Magn Reson Imaging
(1994)
Demonstration of increased myocardial lipid with postischemic dysfunction (”myocardial stunning”) by proton nuclear magnetic resonance spectroscopy
J Am Coll Cardiol
Ischemia and reperfusion induced multilamellar vesicles in isolated rabbit hearts: Time correlation between morphometric data and metabolic alterations
J Mol Cell Cardiol
Mitochondrial physiology and pathology; concepts of programmed cell death of organelles, cells and organisms
Mol Aspects Med
Immunology today
Phospholipid binding of annexin V: Effects of calcium and membrane phosphatidylserine content
Arch Biochem Biophys
Evaluation of annexin V as a platelet directed thrombus targeting agent
Thromb Res
Phospholipid binding properties of human placental anticoagulant protein I, a member of the lipocortin family
J Biol Chem
The use of technetium Tc 99m annexin V for in vivo imaging of apoptosis during cardiac allograft rejection
J Thorac Cardiovasc Surg
Oxygen stress induces an apoptotic cell death associated with fragmentation of mitochondrial genome
Biochemical and Biophysical Research Communications
The application of NMR spectroscopy to the study of apoptosis
Cell Mol Biol
Dying a thousand deaths: Radionuclide imaging of apoptosis
Q J Nucl Med
In vivo detection and imaging of phosphatidylserine expression during programmed cell death
Proc Natl Acad Sci U S A
Imaging brain tumor proliferative activity with [124I] iododeoxyuridine
Cancer Res
Quantification of apoptotic cells with fluorescein isothiocyanate labeled annexin V in Chinese hamster ovary cell cultures treated with cisplatin
Cytometry
Placental DNA pregnancy proteins
Diffusion-weighted interleaved echo-planar imaging with a pair of orthogonal navigator echoes
Magnetic Resonance in Medicine
Monitoring early response of experimental brain tumors to therapy using diffusion magnetic resonance imaging
Clin Cancer Res
Combined effect of tumor necrosis factor-related apoptosis-inducing ligand and ionizing radiation in breast cancer therapy
Proc Natl Acad Sci U S A
High frequency continuous wave Doppler ultrasound system for the detection of blood flow in the microcirculation
Ultrasound Med Biol
Ultrasound imaging of apoptosis: High-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo
Br J Cancer
In vivo measurement of water diffusion in the human heart
Magnetic Resonance in Medicine
Cited by (12)
Amyloid Imaging: From Benchtop to Bedside
2005, Current Topics in Developmental BiologyCitation Excerpt :They have also become an indispensable tool in the drug discovery process to bridge the gap between laboratory research and clinical applications. Recent examples include evaluation of tumor metabolism and proliferation (Brock et al., 2000; Shields et al., 1998a), drug receptor interaction (Anderson et al., 2001; Hume et al., 1997; Kapur et al., 1997a,b,c), modulation of multidrug resistance (Chen et al., 1997; Hendrikse et al., 1999a,b; Levchenko et al., 2000; Vecchio et al., 1997), quantitating angiogenensis and antivascular activity (Vavere and Lewis, 2003; Weber et al., 2001), detection of apoptosis (Blankenberg and Strauss, 2001; Blankenberg et al., 2001; Narula et al., 2001), tumor hypoxia (Casciari et al., 1995; Foo et al., 2004; Lewis et al., 2002), therapy response, and gene expression (Blasberg, 2002; Blasberg and Gelovani‐Tjuvajev, 2002; Dehdashti et al., 1999; Gambhir et al., 1999; Herschman, 2004; Shields et al., 1998b; Walker and Zigler, 2003; Walker et al., 2004). In AD, anti‐amyloid therapies are currently under development to halt or reverse the progressive accumulation of Aβ deposit in AD brain.
Molecular (functional) imaging for radiotherapy applications: An RTOG symposium
2003, International Journal of Radiation Oncology Biology PhysicsImaging Myocardial Ischemia and Reperfusion Injury via Cy5.5-Annexin V
2012, Nuclear Medicine and Molecular ImagingEffect of asiaticoside on <sup>99m</sup>Tc-tetrofosmin and <sup>99m</sup>Tc-sestamibi uptake in MCF-7 cells
2011, Journal of Nuclear Medicine TechnologyPET radiopharmaceuticals in nuclear cardiology: Current status and limitations
2009, Integrating Cardiology for Nuclear Medicine Physicians: A Guide to Nuclear Medicine Physicians
Address reprint requests to H. William Strauss, MD, Division of Nuclear Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Room H0101, Stanford, CA 94305, e-mail: [email protected]
Supported in part by National Heart, Lung, and Blood Institute (NHLBI) grant number HL61717-01.