Skip to main content

Advertisement

SpringerLink
Log in

Small Animal Imaging Center Design: The Facility at the UCLA Crump Institute for Molecular Imaging

  • Research Article
  • Published:
Molecular Imaging and Biology Aims and scope Submit manuscript

Abstract

Purpose

The growing number of mouse and rat experiments, coupled with advances in small-animal imaging systems such as microPET®, optical, microCAT™, microMR, ultrasound and microSPECT, has necessitated a common technical center for imaging small animals.

Procedures

At the UCLA Crump Institute for Molecular Imaging, we have designed and built a facility to support the research interests of a wide range of investigators from multiple disciplines. Requirements to satisfy both research and regulatory oversight have been critically examined. Support is provided for investigator training, study scheduling, data acquisition, archiving, image display, and analysis.

Results

The center has been in operation for more than 18 months, supporting more than 13,000 individual imaging procedures.

Conclusions

We have created a facility that maximizes our resource utilization while providing optimal investigator support, as well as the means to continually improve the quality and diversity of the science by integrating physical and biological sciences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Rice BW, Cable MD, Nelson MB (2001) In vivo imaging of light-emitting probes. J Biomed Opt 6:432–440

    Article  PubMed  CAS  Google Scholar 

  2. Phelps ME (2000) PET: The merging of biology and imaging into molecular biology. J Nucl Med 41:661–681

    PubMed  CAS  Google Scholar 

  3. Chatziioannou AF (2002) PET scanners dedicated to molecular imaging of small animal models. Mol Imaging Biol 4:47–63

    Article  PubMed  Google Scholar 

  4. Tai C, Chatziioannou AF, Siegel S, Young J, Newport D, Goble RN, Nutt RE, Cherry SR (2001) Performance evaluation of the microPET P4: A PET system dedicated to animal imaging. Phys Med Biol 46:1845–1862

    Article  PubMed  CAS  Google Scholar 

  5. Paulus MJ, Gleason SS, Kennel SJ, Hunsicker PR, Johnson DK (2000) High resolution X-ray computed tomography: An emerging tool for small animal cancer research. Neoplasia 2:62–70

    Article  PubMed  CAS  Google Scholar 

  6. Troy T, Jekic-McMullen D, Sambucetti L, Rice BW (2004) Quantitative comparison of the sensitivity of detection of fluorescent and bioluminescent reporters in animal models. Mol Imaging 3:9–23

    Article  PubMed  CAS  Google Scholar 

  7. Stout DB, Chow PL, Gustilo A, Grubwieser S, Chatziioannou AF (2003) Multimodality isolated bed system for mouse imaging experiments. Mol Imaging Biol 5:128–129

    Google Scholar 

  8. Fueger BJ, Tran C, Mellinghoff IK, Halpern BS, Bodenstein CH, Stout DB, Phelps ME, Czernin J, Weber WA (2005) Optimizing PET imaging with 2-deoxy-2[18Fluoro]-d-glucose in SCID mice. Mol Imaging Biol 7 (in press)

  9. Szczesny G, Velhelmann A, Massberg S, Nolte D, Messmer K (2004) Long-term anesthesia using inhalatory isofluorane in different strains of mice—the haemodynamic effects. Lab Anim 38:64–69

    Article  PubMed  CAS  Google Scholar 

  10. Gambhir SS, Barrio JR, Wu L, Iyer M, Namavari M, Satyamurthy N, Bauer E, Parrish C, MacLaren DC, Borghei AR, Green LA, Sharfstein S, Berk AJ, Cherry SR, Phelps ME, Herschman HR (1998) Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir. J Nucl Med 39:2003–2011

    PubMed  CAS  Google Scholar 

  11. Truong D, Stout DB, Yang C, Chang A, Rannou F, Gambhir SS, Huang SC, Phelps ME, Chatziioannou AF (2002) Multisystem data management for small animal imaging. Mol Imaging Biol 4:S28

    Google Scholar 

  12. Shattuck DW, Rapela J, Asma E, Chatzioannou AF, Qi J, Leahy RM (2002) Internet2-based 3D PET image reconstruction using a PC cluster. Phys Med Biol 7:2785–2795

    Article  Google Scholar 

  13. Loening AM, Gambhir SS (2003) AMIDE: A free software tool for multimodality medical image analysis. Mol Imaging 2:131–137

    Article  PubMed  Google Scholar 

  14. Truong DC, Huang S-C, Hoh C, Vu D, Gambhir SS, Phelps ME (1998) A Java/Internet-based platform independent system for nuclear medicine computing. J Nucl Med 39:278P

    Google Scholar 

  15. Huang SC, Truong DC, Wu HM, Chatziioannou AF, Wu A, Phelps ME (2004) A “kinetic imaging system (KIS)” for microPET. Mol Imaging Biol 6:79

    Google Scholar 

  16. Jacobson MS, Hung JC, Mays TL, Mullan BP (2002) The planning and design of a new PET radiochemistry facility. Mol Imaging Biol 4:119–127

    Article  PubMed  Google Scholar 

  17. Blasberg RG (2003) In vivo molecular imaging: Multimodality nuclear and optical combinations. Nucl Med Biol 8:879–888

    Article  CAS  Google Scholar 

  18. Herschman H (2004) Noninvasive imaging of reporter gene expression in living subjects. Adv Cancer Res 92:29–80.

    Article  PubMed  CAS  Google Scholar 

  19. Kudo T, Fukuchi K, Annala AJ, Chatziioannou AF, Allada V, Dahlbom M, Tai YC, Inubushi M, Huang S-C, Cherry SR, Phelps ME, Schelbert HR (2002) Noninvasive measurement of myocardial activity concentrations and perfusion defect sizes in rats with a new small-animal positron emission tomograph. Circulation 106:118–123

    Article  PubMed  Google Scholar 

  20. Huang SC, Wu HM, Shoghi-Jadid K, Stout DB, Chatziioannou A, Schelbert HR, Barrio JR (2004) Investigation of a new input function validation approach for dynamic mouse microPET studies. Mol Imaging Biol 6(1):34–46 (Jan–Feb)

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We would like to acknowledge the many investigators who have helped design this new imaging facility over the past several years. We thank Judy Edwards, Waldemar Ladno, and Victor Dominguez for their hard work and long hours; David Skorupinski for his support as the construction manager; and the RSO and Biosafety committee members Leslie Hoeffer, Ken Kessler, and Amanda Ogden for their help in making us compliant with the various regulations. We also wish to thank Harvey Herschman for his help and guidance, and Ron Sumida for his insights into streamlining our procedures. Support for this project was provided by the Biological and Environmental Research Division of the Department of Energy (DOE), the National Cancer Institute (NCI). In Vivo Cellular and Molecular Imaging Centers (NIH ICMIC), and Small Animal Imaging Resource Program (SAIRP), the Jonsson Comprehensive Cancer Center UCLA, and the NCI SPORE in Prostate Cancer.

Author information

Authors and Affiliations

  1. Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, The David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    David B. Stout PhD, Arion F. Chatziioannou PhD, Robert W. Silverman BS & Michael E. Phelps PhD

  2. Division of Laboratory Animal Medicine, The David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Timothy P. Lawson DVM

  3. Molecular Imaging Program at Stanford (MIPS), Department of Radiology & Bio-X Program, Stanford University, Stanford, CA, USA

    Sanjiv S. Gambhir MD, PhD

Authors
  1. David B. Stout PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arion F. Chatziioannou PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Timothy P. Lawson DVM
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert W. Silverman BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanjiv S. Gambhir MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Michael E. Phelps PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to David B. Stout PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stout, D.B., Chatziioannou, A.F., Lawson, T.P. et al. Small Animal Imaging Center Design: The Facility at the UCLA Crump Institute for Molecular Imaging. Mol Imaging Biol 7, 393–402 (2005). https://doi.org/10.1007/s11307-005-0015-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11307-005-0015-2

Key words

Search

Navigation