Regular ArticlePKH26 and125I-PKH95: Characterization and Efficacy as Labels forin Vitroandin VivoEndothelial Cell Localization and Tracking
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Injectable adipose-derived stem cells-embedded alginate-gelatin microspheres prepared by electrospray for cartilage tissue regeneration
2022, Journal of Orthopaedic TranslationCitation Excerpt :PKH26 is a lipophilic dye with strong fluorescence that stains membranes. This labelling method has been used to track cell migration in vivo [21]. We used PKH26 dye to label ADSCs and then encapsulated them in Alg-Gel MSs.
Slow-cycling (dormant) cancer cells in therapy resistance, cancer relapse and metastasis
2022, Seminars in Cancer BiologyCitation Excerpt :Live slow-cycling cells, on the other hand, can be enriched and purified via fluorescent dyes that preferentially bind membrane or intracellular proteins (e.g., CellTrace or CFSE) or membrane lipids (e.g., PKH and DiD) (Fig. 3B). Among the first fluorescent dyes used to characterize cells with unique cycling parameters, PKH-based membrane labelling dye was first developed by Paul Horan in 1989 [61] and subsequently used by many to label and track cells with differing proliferative capacities [62,63] or with different proliferative kinetics [64–66] and to label extracellular vesicles [67,68]. Among the lipid-labeling dyes, PKH26 is the most commonly used.
Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury
2014, Experimental Cell ResearchCitation Excerpt :Serum levels of INF-γ and TNF-α were below 30 pg/mL in normal control mice, and T-MSC injection alone does not alter their levels. To confirm whether T-MSCs successfully homed the damaged liver after ConA injection, T-MSCs and HEK 293T cells were stained with the dye PKH26, which is an effective means of labelling live cells for in vivo tracking [29,30]. PKH26-stained T-MSCs, but not stained HEK293T cells, were detected in damaged liver tissues (Fig. 4).
Valproic acid enhances the effect of bone marrow-derived mononuclear cells in a rat ischemic stroke model
2014, Brain ResearchCitation Excerpt :The numbers of positively stained cells were counted in three randomly selected microscopic fields under 100× magnification in a blind fashion. To track the transplanted MNCs in the ischemic brain, PKH26, a red fluorescent cell linker, was used (Ford et al., 1996). MNCs were harvested and labeled with PKH26 as described previously (Kamiya et al., 2008).
Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells
2014, Journal of Bioscience and Bioengineering