Comparison of Standard and Alternative RCP Testing Methods
ITLC-SG (n = 3) | |||
Mobile phase | Development distance (cm) | Time (min) | Rs |
Methanol:1 M ammonium acetate | |||
50:50 | 10 | 25.3 ± 1.7 | 2.3 |
9 | 21.2 ± 0.3 | 1.6 | |
8 | 16.1 ± 0.3 | 1.5 | |
7 | 13.3 ± 0.2 | 1.5 | |
80:20 | 10 | 18.6 ± 0.4 | 2.1 |
9 | 15.4 ± 0.2 | 1.5 | |
8 | 12.7 ± 0.2 | 1.4 | |
7 | 10.3 ± 0.2 | 0.8 |