Technologist Checklist for 177Lu-DOTATATE Preparation
| Tech initials | Step |
| Protect cart with chux pads | |
| Hang 250-mL saline bag; prime tubing with saline | |
| Connect tubing to 3-way stopcock; prime 3-way stopcock with saline | |
| Prime microbore tubing with saline; connect microbore tubing to 3-way stopcock | |
| Load tubing into Graseby pump | |
| Set rate on Graseby pump (50 mL/h); clear volume; change dose calibrator setting, sleeve, and plunger to those for 177Lu | |
| Label syringe with patient’s name, date of birth, medical record number, drug, dose, and date | |
| Assay vial (place gauze in bottom of pig while vial is still in dose calibrator), and record result and time; if less than 6.66 GBq or 180 mCi (7.4 GBq or 200 mCi − 10%), contact nuclear medicine physician before proceeding | |
| Draw entire dose from vial into 30-mL syringe | |
| Vent dose vial by attaching 20-gauge needle to 1-mL syringe (plunger removed) and inserting at tilted angle just enough to puncture vial | |
| Draw dose by attaching 3.5-in (8.9-cm) spinal needle to 30-mL shielded syringe, inserting into vial (tilted on wedge) until reaching its bottom, and slowly withdrawing entire volume; if withdrawal is difficult, slightly loosen thumbscrew of shield (2.5-in [6.3-cm] needle can be used but will not reach bottom) | |
| Before removing syringe, use tongs to raise vial and assess whether entire volume has been withdrawn | |
| Slowly pull syringe straight up and out of vial | |
| Pull back plunger to ensure that no liquid remains in hub of needle | |
| Discard spinal needle into sharps waste bucket; attach regular needle | |
| Remove air from syringe | |
| Assay residual activity in vial, and record result and time | |
| Assay syringe dose, and record result and time (nuclear medicine physician to confirm) | |
| Connect syringe dose to 3-way stopcock and load into Graseby pump | |
| After infusion, assay residual activity in syringe and needle |