RCP Evaluation Outcomes with Various Mobile Phases
| ITLC-SG paper | ||||
| Mobile phase | Impurity Rf | 68Ga-DOTATATE Rf | Development time (min) (n = 3) | Rs |
| Methanol:1 M ammonium acetate | ||||
| 50:50 | 0.0–0.1 | 0.8–1.0 | 25.3 ± 1.7 | 2.3 |
| 60:40 | 0.0–0.1 | 0.8–1.0 | 23.9 ± 0.5 | 2.2 |
| 80:20 | 0.0–0.1 | 0.6–0.9 | 18.6 ± 0.4 | 2.1 |
| 90:10 | 0.0–0.1 | 0.3–0.6 | 15.1 ± 0.3 | 0.6 |
| 95:5 | 0.0–0.1 | 0.1–0.3 | 13.9 ± 0.3 | NA |
| 100:0 | 0.0–0.1 | 0.0–0.1 | 10.3 ± 0.2 | NA |
| 0:100 | 0.0–0.1 | 0.0–0.1 | 10.3 ± 0.6 | NA |
NA = unable to identify 2 peaks on ITLC-SG.