Comparison of SPE Methods for Determination of RCP of 99mTc-MAG3
| Step | European method | American method |
|---|---|---|
| Preparation | 10 mL of ethanol, 10 mL of HCl at 1 mmol/L, and 5 mL of air | 10 mL of ethanol, 10 mL of HCl at 1 mmol/L, and 5 mL of air |
| Solution A (hydrophilic impurities) | 5 mL of HCl at 1 mmol/L and 5 mL of 0.5% ethanol-phosphate buffer | 10 mL of HCl at 1 mmol/L |
| Solution B (99mTc-MAG3) | 10 mL of 7% ethanol-phosphate buffer | 10 mL of 1:1 ethanol:0.9% saline |
| Lipophilic impurities + colloid | 10 mL of ethanol | Residue counted |