Sodium Chromate 51Cr Injection USP Prescribing Information Radiolabeling Procedure
| Step | Procedure |
|---|---|
| 1 | Whole blood (30–50 mL) is added to sterile vial containing ACD solution (5–10 mL) with gentle mixing |
| 2 | 1.85–7.4 MBq (50–200 μCi) of 51Cr sodium chromate are added to vial, and whole-blood suspension is incubated at room temperature with frequent gentle mixing (swirling) |
| 3 | Red blood cell labeling is terminated with addition of 50–100 mg of ascorbic acid for injection USP |
| 4 | 51Cr whole blood is centrifuged, and supernatant (plasma) radioactivity is removed |
| 5 | 51Cr red blood cells are resuspended in normal saline solution USP to known volume |
| 6 | Red blood cells are assayed in dose calibrator and reinjected into patient |
ACD = acid citrate dextrose.