International Committee for Standardization in Haematology 51Cr Radiolabeling of Red Blood Cells
| Step | Procedure |
|---|---|
| 1 | Whole blood (10 volumes) is added to NIH ACD-A solution (1–5 volumes) |
| 2 | Cell suspension is centrifuged gently (∼1,000 g) for 5 min |
| 3 | Supernatant is removed and discarded |
| 4 | 51Cr sodium chromate (7.4 kBq/kg of body weight) in volume ≥ 0.2 mL is added and mixed gently |
| 5 | Incubation is performed for 15 min at 37°C |
| 6 | 51Cr-labeled cells are washed twice in 4–5 volumes of isotonic saline |
| 7 | Cells are resuspended in volume of isotonic saline to provide infusion volume of 10 mL |
NIH ACD-A = National Institutes of Health acid citrate dextrose A.