TY - JOUR T1 - Rapid Instant Thin-Layer Chromatography System for Determining the Radiochemical Purity of <sup>68</sup>Ga-DOTATATE JF - Journal of Nuclear Medicine Technology JO - J. Nucl. Med. Technol. SP - 285 LP - 287 DO - 10.2967/jnmt.117.200873 VL - 46 IS - 3 AU - Michael G. Bornholdt AU - Kayla M. Woelfel AU - Ping Fang AU - Mark S. Jacobson AU - Joseph C. Hung Y1 - 2018/09/01 UR - http://tech.snmjournals.org/content/46/3/285.abstract N2 - The objective of this study was to develop instant thin-layer chromatography (ITLC) conditions for the determination of radiochemical purity of 68Ga-DOTATATE in a shorter time period than those stated in the NETSPOT (Advanced Accelerator Applications, Saint-Genis-Pouilly, France; AAA) kit package insert (PI). A faster ITLC system is needed to reduce the 48- to 50-min development time so that more radioactivity is available for single patient use and wait times are shorter in the event of kit failure. Methods: Variations of the PI mobile system were evaluated with microfiber chromatography paper impregnated with silica gel (ITLC-SG). After a more suitable mobile system was identified, evaluation began by attempting to shorten the 10-cm development distance to 7, 8, and 9 cm. Results: Experiments using variations of PI mobile phase showed that increasing the proportion of methanol in the mobile phase decreased development time. Additionally, if the ratio of 1 M ammonium acetate was reduced to 10% or less, retention factor values fall outside specification. Reducing the development distance shortened development time as expected; however, it also affected the resolution aspect of the radiochromatogram. Conclusion: The fastest developing ITLC system, which maintained resolution and peak shape, was methanol:1 M ammonium acetate (80:20 V/V) with ITLC-SG using a development distance of 8 cm. ER -