@article {Luebke259, author = {Angela L. Luebke and Douglas M. Wilary and Douglas W. Mahoney and Joseph C. Hung}, title = {Evaluation of an Alternative Radiochemical Purity Testing Method for Technetium-99m-Sestamibi}, volume = {28}, number = {4}, pages = {259--263}, year = {2000}, publisher = {Society of Nuclear Medicine}, abstract = {Objective: Our study evaluated the accuracy and reliability of 3 radiochemical purity (RCP) measurement methods of 99mTc-sestamibi. A regular-sized (1.0 cm {\texttimes} 9.0 cm) Whatman 31 ET Chr paper strip (regular 31 ET) also was included in our evaluation because of its ease in handling. Methods: The miniaturized and regular 31 ET methods were compared with the standard RCP testing method (aluminum oxide{\textendash}coated plastic thin-layer chromatography [TLC] plate, with >= 95\% ethanol as the developing solvent). A total of 30 experimental runs were performed in triplicate (n = 90) over an RCP range of 82\%{\textendash}98\%. The 99mTc-sestamibi preparations were reconstituted purposely to ensure that 50\% of the tested samples had RCP values below the 90\% limit. Results: The evaluated RCP ranges were 89.9\% {\textpm} 6.3\%, 91.0\% {\textpm} 3.8\%, and 91.4\% {\textpm} 4.3\% for the TLC, miniature 31 ET, and regular 31 ET methods, respectively (n = 30 each). A strong correlation was found between the TLC and miniature 31 ET methods (r = 0.92), as well as between the TLC and regular 31 ET methods (r = 0.94). Both alternative methods tended to overestimate RCP value as determined by the TLC method, especially in an RCP range below 95\%. This resulted in a false-positive rate of 27\% for the miniature 31 ET method and 33\% for the regular 31 ET method. The test/retest reliability was 99\% for both the TLC and regular 31 ET methods, and 91\% for the TLC and miniature 31 ET methods. Conclusion: The miniature and regular 31 ET methods produced a high false-positive rate, which makes them unacceptable for the determination of RCP value of 99mTc-sestamibi.}, issn = {0091-4916}, URL = {https://tech.snmjournals.org/content/28/4/259}, eprint = {https://tech.snmjournals.org/content/28/4/259.full.pdf}, journal = {Journal of Nuclear Medicine Technology} }