In the past few years, the development of sequencespecific DNA nucleases has progressed
rapidly and such nucleases have shown their power in generating efficient targeted …

Background The CRISPR/Cas9 system, composed of a single-guide RNA for target recognition
and a Cas9 protein for DNA cleavage, has the potential to revolutionize agriculture as …

Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases (ZFNs)(Carroll,
2011), Transcription Activator-Like Effector Nucleases (TALENs)(Mahfouz et al., 2011; …

Arabidopsis thaliana HYPONASTIC LEAVES1 (HYL1) is a microRNA (miRNA) biogenesis
protein that contains two N-terminal double-stranded RNA binding domains (dsRBDs), a …

CRISPR /Cas9 and TALEN are currently the two systems of choice for genome editing. We
have studied the efficiency of the TALEN system in rice as well as the nature and inheritability …

The CRISPR / C as9 system has been demonstrated to efficiently induce targeted gene
editing in a variety of organisms including plants. Recent work showed that CRISPR / C as9‐…

The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated)
system has emerged as a powerful tool for targeted gene editing in many organisms, …

The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9
system has been used successfully for simultaneous modification of multiple loci in plants. …

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein
9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …

Rice (Oryza sativa) is the staple food for more than half of the world’s population. Technologies
enabling precise and efficient DNA knock-in or replacement, hereinafter referred to as KI, …