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Journal of Nuclear Medicine Technology Volume 34, Number 3, 2006 174-178
© 2006 by Society of Nuclear Medicine

Rapid Determination of the Radiochemical Purity of 99mTc-Antimony Trisulfide Colloid Prepared by Standard and Alternative Heating Methods

Douglas R. Smyth

RAH Radiopharmacy, Department of Nuclear Medicine, Royal Adelaide Hospital, Adelaide, South Australia, Australia

Correspondence: For correspondence or reprints contact: Douglas R. Smyth, PhD, RAH Radiopharmacy, Department of Nuclear Medicine, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia 5000, Australia. E-mail: dsmyth{at}mail.rah.sa.gov.au

The purpose of this study was to validate a rapid quality control method for the lymphoscintigraphic tracer 99mTc-antimony trisulfide colloid (99mTc-ATC). Methods: ATC was labeled with 99mTc according to the manufacturer's instructions as well as by alternative heating conditions designed to provide a range of percentages of radiochemical purity (RCP): the tracer was prepared in a dry block heater with heating cavities of different sizes, the temperature of the heating block was varied from 70°C to 115°C, or the duration of heating was varied from 15 to 35 min. Anion-exchange minicolumns were trialled to separate any 99mTc-pertechnetate impurity from 99mTc-ATC with physiologic saline as the eluent. Quality control results were compared with the results from the manufacturer's recommended method, which uses an instant thin-layer chromatography (ITLC) strip with saline as the migrating solution. Results: The quality control results obtained with a cartridge method in 2–3 min compared favorably with those obtained with the ITLC method with saline when the tracer was prepared by heating at 115°C in a dry block heater for 35 min (RCPs, 99.4% ± 0.3% [mean ± SD] and 99.2% ± 0.3%, respectively; n = 25). The cartridge and ITLC quality control results also were in excellent agreement (correlation coefficient, 0.99) over a range of RCPs (80%–100%). An alternative anion-exchange cartridge that was tested in this study was not suitable for assaying the RCP of 99mTc-ATC because of the complete retention of 99mTc-pertechnetate on the sorbent. Conclusion: Compared with the established ITLC method, the cartridge quality control method tested in this study is rapid and provides a reliable assessment of the RCP of 99mTc-ATC. For the preparation of 99mTc-ATC, a dry block heater can be successfully substituted for a boiling water bath and is recommended for heating at high altitudes.

Key Words: antimony trisulfide colloid; radiocolloid; quality control; radiochemical purity; cartridge






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