HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Norenberg, J. P. Articles by Carroll, T. R. Search for Related Content PubMed PubMed Citation Articles by Norenberg, J. P. Articles by Carroll, T. R.

The Effect of Selected Preparation Variables on the Radiochemical Purity of ^99mTc-Sestamibi

¹Radiopharmaceutical Sciences Program, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, New Mexico;
²Groton Labs, Pfizer Global Research and Development, Groton, Connecticut;
³Departments of Family Practice and Epidemiology, School of Medicine, Michigan State University, East Lansing, Michigan;
⁴College of Pharmacy, University of New Mexico Health Sciences Center;
⁵DuPont-Merck Pharmaceutical Co., Inc., Billerica, Massachusetts

Objective:Measurement of cardiac perfusion via agents such as ^99mTc-sestamibi (Cardiolite; DuPont-Merck Pharmaceutical Co., Inc.) is widely used in clinical nuclear medicine for the diagnosis of coronary artery disease. The monograph for ^99mTc-sestamibi recommends at least 90% radiochemical purity (RCP) for clinical use. Various factors may influence the RCP of certain reagent kits. Some of these include the amount of activity added to the reagent kit, the generator ingrowth time, the generator manufacturer, the age of the eluate, and the age of the formulated kit. A D-optimal design with a 20-experiment run was devised to study the effects of these variables either alone or in combination on the RCP of ^99mTc-sestamibi.

Methods:The RCP was assessed by Baker-Flex thin-layer and high-performance liquid chromatographic methods, immediately and 6 h after reconstitution of the ^99mTc-sestamibi.

Results:The results showed that 4 of the 5 variables studied were statistically significant predictors of the RCP. The age of the formulated kit did not influence the RCP.

Conclusion:For any combination of these 4 variables, the mean RCP remained greater than or equal to 90%, that is, within the recommended range of RCP for clinical use at radioactivity levels ranging from 5,550 MBq to 37,000 MBq.

Key Words: ^99mTc-sestamibi; Cardiolite; coronary artery disease; radiochemical purity